Chloroquin extends contour length of dna

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  1. nicant Moderator

    Chloroquin extends contour length of dna

    The contour length of a polymer chain (a big molecule consisting of many similar smaller molecules) is its length at maximum physically possible extension. We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising.

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    Characterizing the Interaction Between DNA and. YO-PRO-1 stacks between the base pairs and extends the contour length of DNA, changing the electric charge and the persistence length of DNA. Improve DNA backbone contour length estimates by inputting accurate estimates of curvature to a length estimator that models the DNA contour as a sequence of straight lines and circular arcs 42, 32, 21. 1.4 Our contribution Our software system uses the image processing pipeline of Cirrone 5 and extends the length estimation pipeline in a. Et al. 1972. The present study confirms and extends our earlier results DNA from 4-cell stages o lumbricoidesf A. with a density of 1-683 consist6 gem" s of circular molecules with a contour length of 4-64 ± 0-03 /tm. Electron microscopy further demonstrates the presence of either supercoiled, loosely twisted, or open circles. The

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    Chloroquin extends contour length of dna

    Real-time imaging of DNA loop extrusion by condensin Science, Measuring biomolecules an image processing and length.

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  5. The dissociation constant of the DNA-chloroquine complex varied from 27 microM to 2.6 mM, depending on the ionic strength of the buffer. Our results suggest that chloroquine binding to DNA is highly dependent on salt concentration.

    • Binding of chloroquine to DNA..
    • Evidence of Strain Hardening in DNA Gels.

    Contour length is a term used in molecular physics. The contour length of a polymer chain a big molecule consisting of many similar smaller molecules is its length at maximum physically possible extension. Contour length is equal to the product of the number of segments of polymer moleculen and its lengthl. Fig. 2 demonstrates this effect for a complex of DNA with chloroquine. The s0 of the DNA preparation decreased from 21.6 to 18.6 upon formation of the chloroquine complex. The experiments recorded in Figs. Model 8–10, from which a persistence length, P, and a contourlength,L o,canbeobtained. Between10and65pN,the molecule deviates from the predictions of the inextensible WLC as it extends beyond its B-form contour length. From this ‘‘enthalpic elasticity’’ regime an elastic stretch modulus, S, can be obtained.

  6. malkari Moderator

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  7. Magnus33 Moderator

    Chloroquine Explained Mechanism of action Antimalarial Inside red blood cell s, the malarial parasite, which is then in its asexual lifecycle stage, must degrade hemoglobin to acquire essential amino acids, which the parasite requires to construct its own protein and for energy metabolism.

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